This software is a general data reduction package used to analyze data generated from colorimetric. Fluorescence was measured in relative light units rlus using a 96well black flatbottomed plate corning costar, cambridge, mass. Kc 4 data analysis software specifications techylib. The software is a big upgrade from the kc4 that biotek had. Samples were read using an fl600 fluorescence plate reader with reader function controlled by kc4 data reduction software on an external pc.
It is computercontrolled using bioteks gen5 or kc4 pc software for all operations including data reduction and analysis. On the linux pcs midas, iraf, idl, p2pp, gv, gimp, firefox and more are installed. The reading was performed at an excitationemission wavelength of 485 to 520 nm using kc4 data reduction software biotek instruments, winooski, vt. This evaluation must include the confirmation that performance characteristics for the specific. Gen5 microplate reader and imager software from biotek. Intended use statement the elx808 is an absorbance microplate reader. Parameters such as read type, filter pairs, probe selection. Bio tek powerwave x 340 microplate reader w kc4 complete for sale. As seen in figure 4, when the absorbance at 260 nm of a serial dilution of dna is.
Thorough research of the regulation and feedback from key customers provided a. Helicobacter pylori igg antibodies in serum by enzyme. Customizable well identifiers fully customizable well identifiers per protocol sample identification manual entrybarcode entry sample name import from text file sample name list can be printed for use as pipetting guide changemaskcreate data yes, kc4. The recognition of high molecular weight melanoidins as. Data handling and reduction is performed using bio tek kc4 kineticalc for windows.
Fluorescencebased total protein determination using the. Reports and export data are customizable column and matrix reports standard curve can be reported exclusive powerreportstm feature uses microsoft wordexcel for total report customization. Or, you can instantly analyze your linearity and calibration. Powerwave series or the synergy ht series readers that require kc4 data reduction software running on a remote pc and do not have keypad programming remote pc biostack controlling software is. Kc4 software program biotek instruments bioz ratings for life. The color was allowed to develop for 10 minutes at room temperature and the absorbance at 540 nm was then determined using a powerwave 200 scanning microplate spectrophotometer with kc4 data. Kineticalc ii software for microplate reader control and data reduction. Kc4 data analysis software, supplied by biotek instruments, used in various. An extensive menu of data reduction, standard curve fits.
Pc running kc4 data reduction software biotek instruments, winooski, vt. Kc3 data is collected from a software system coded in java, containing 458 modules. Data was collected using kc4 data reduction software, exported to microsoft excel. Characterization of excitation and emission spectra of. Pdf fluorimetric quantitation of protein using the. Frontiers reexamining the role of hydrogen peroxide in. Amino acid fortified diets for weanling pigs replacing. Ion channel assay development on the synergy ht multi. Below is some detail information on the data reduction tools available. The use of kc4 software to control the reader allows the user a great deal of flexibility in regards to data reduction capabilities. Data reduction is the transformation of numerical or alphabetical digital information derived empirically or experimentally into a corrected, ordered, and simplified form. Kc4 data reduction software biotek instruments, a 4parameter nonlinear equation describing the standard curve was generated. As seen in figure 4, when the absorbance at 260 nm of a serial dilution of dna is measured, a linear regression analysis can be used to best describe the data.
Kc4 data reduction software was used to control reader function. For this data set, the linear regression analysis was performed using the kc4 tm data reduction software that is bundled with the plate reader the total protein concentration of each sample is used to. The software allows the user to define any configuration of. Gen5 offers unsurpassed data reduction capability as well as customizable data output options. Kc4 signature is the first microplate data reduction software to be totally compliant to the fda regulation 21 cfr part 11. Kc4 data reduction software was used to control the reader, collect the data and perform data analysis. Data reduction software free download data reduction. Using synergy ht multimode microplate reader to measure time. Top 4 download periodically updates software information of data reduction full versions from the publishers, but some information may be slightly outofdate using warez version, crack, warez passwords, patches, serial numbers, registration codes, key generator, pirate key, keymaker or keygen for data reduction license key is illegal.
Data objects from kc4 are embedded into an excel sheet or word document, customized, then saved into kc4 protocol. When standalone use is preferred, the elx800 onboard software is extensive for a variety of qualitative and. The absorbance detection optics uses a xenon flash lamp and monochromator for filterfree wavelength selection from. Kc4 data reduction software biotek instruments inc. In february 2011, the tanker was selected by the united states air. Even new data reduction steps can be inserted into the pipeline. Gen5 is software designed for the users of microplate instrument technology, to aid and assist them in gathering, analyzing and creating reports from collected data. Using kc4 data reduction software biotek instruments, a 4parameter logistic fit equation describing the standard curve was generated. Top 4 download periodically updates software information of data reduction full versions from the publishers, but some information may be slightly outofdate using warez version, crack, warez. Using kc4 data reduction software biotek instruments winooski vt a 4parameter logistic bestfit equation describing the standard curve can be. Measurements of potassium dichromate k2cr2o7 demonstrate the spectral scanning feature of the synergy ht. The role of the data reduction software is to control reader functions such as wavelengths selection for endpoint determinations, reading interval for kinetic.
Using kc4 data reduction software to perform data reduction, several different curve fits can be used to best fit the data. Limited availability of fish meal and whey protein concentrate increases overall feed costs. Green fluorescent protein quantification in microplates. Developpers of sumo, dumo, kcleaner and other powerful system utilities. The boeing kc46 pegasus is a military aerial refueling and strategic military transport aircraft developed by boeing from its 767 jet airliner. Galactosidase activity determination using a biotek microplate. Gen5 software is the most advanced on the microplate instrument market today, and is an indispensable tool for microplate users. Designing plasmas for chronic wound disinfection iopscience. Using two filter sets, the synergy was set up to rapidly switch.
The performance characteristics of the data reduction software have not been established with any laboratory. The software allows the user to define any configuration of plate map necessary. Multiuser password login in kc4 signature see protocoldata. The software algorithm and data reduction reports are identical to the data reduction services provided by lgc. Data objects from kc4 are embedded into an excel sheet or word document, customized, then saved into kc4. Or, you can instantly analyze your linearity and calibration verification results with our userfriendly data reduction software program, msdrx. Powerwave series or the synergy ht series readers that require kc4 data reduction software running on a remote pc and do not have keypad programming remote pc biostack controlling software is used to program the biostack. Elx50 microplate strip washer and kc4 data reduction software for windows. The software architecture uses an approach, in which the structure of the reduction pipeline and the available algorithms and tools are fully configurable.
Intelligence system for software maintenance severity. This means that an experienced user can define and implement new data reduction algorithms. While several options are available to reduce background fluorescence. The fluorescence of the formed product, resorufin, was measured at 530 nm excitation and a 590 nm emission using the synergy ht molecular devices, biotek instruments, winooski, vt, usa multidetection microplate reader, and the doseresponse curves were generated using the kc4. Data reduction transformations and customizable validation and cutoff calculations, are all easily defined within the protocol. Fluorescence was determined using either a 485 nm, 20 nm.
Gen5 data analysis software is developed and introduced to run all biotek readers. Gen5 is a unique combination of power and easeofuse that. The resultant raw data, as well as the pathlength corrected data, were plotted using kc4 data reduction software and linear regression analysis was performed. Dawn, the data analysis workbench, is an eclipse based application for scientific data analysis mainly developed at the diamond light source. Performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. Data reduction software max planck institute for radio. Screenshot from kc4 data reduction software depicting typical reading parameters for vsp. Screenshot from kc4 data reduction software depicting typical reading parameters for vsp assays. The user must evaluate this instrument and pcbased software in conjunction with the specific assay. The software of this unit is designed for assay versatility, allowing simple setup menus for injection timing and kinetic read modes. Both solutions were protected from light prior to use. Biotek elx800 community, manuals and specifications.
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